rabbit anti human lilrb4 antibody (Proteintech)
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Proteintech
rabbit anti human lilrb4 antibody
Rabbit Anti Human Lilrb4 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+human+lilrb4/LILRB4+Antibody/pmc12716741-83-24-23
Average 92 stars, based on 5 article reviews
Rabbit Anti Human Lilrb4 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+human+lilrb4/LILRB4+Antibody/pmc12716741-83-24-23
Average 92 stars, based on 5 article reviews
rabbit anti human lilrb4 antibody - by Bioz Stars,
2026-09
92/100 stars
Images
1) Product Images from "The combination of LILRB4-targeting NK cell engagers and cGAS–STING agonists enhances the anti–multiple myeloma immune activity of NK cells"
Article Title: The combination of LILRB4-targeting NK cell engagers and cGAS–STING agonists enhances the anti–multiple myeloma immune activity of NK cells
Journal: PLOS One
doi: 10.1371/journal.pone.0339375
Figure Legend Snippet: (A) Uniform manifold approximation and projection (UMAP) dimensionality reduction clustering identifies multiple cell subpopulations of multiple myeloma cells. (B) The Volcano plots display the gene expression profiles of each tumor cell subpopulation with significant differences compared to other subpopulations. Log2 (fold change) > 1 and adjusted P < 0.05 were considered as significantly differentially expressed genes. (C) Heatmap showing precise differences in LILRB4 expression across tumor cell subsets. (D) Density plot of LILRB4 expression in tumor cells from UMAP. Color intensity indicates the expression level. (E) Copy Number Variation Score for Tumor Subpopulation Cells. (F) Metabolic pathway enrichment analysis of differentially expressed genes in tumor cell subsets, where color intensity reflects the degree of correlation. (G) Dotplot diagrams display the expression of ligand–receptor pairs between LILRB4 + MM cells (the initiating point of communication) and other cells. (H) LILRB4-related survival analysis in MM was performed using the TCGA database, with P values calculated by the log-rank test. (I) Western blot analysis was used to detect LILRB4 protein expression levels in MM1.S and RPMI-8226 cells. (J) Flow cytometry was employed to assess the expression of LILRB4 protein in MM1.S and RPMI-8226 cells.
Techniques Used: Gene Expression, Expressing, Western Blot, Flow Cytometry
Figure Legend Snippet: (A) Schematic representation of the BiKE structure. (B) Schematic representation of BiKE-mediated NK cells killing of tumors. (C) BiKE SDS-PAGE of non-reduced and reduced proteins, stained with Coomassie blue. (D) ELISA test measuring the affinity of different concentrations of BiKE to the LILRB4 antigen. (E) Elisa tests the affinity of different concentrations of BiKE to the CD16A antigen. (F) Indirect labeling Flow cytometry was used to assess the binding ability of BiKE to MM1.S, NK, and K562 cells. Goat anti human IgG (H + L) antibody labeled with FITC as the secondary antibody. (G) PBMCs were co-cultured with LILRB4 + MM1.S cells for 4 hours. The regulation of CD69 expression in NK cells by monoclonal antibodies and BiKE in the co-culture system was analyzed by flow cytometry. Data are presented as mean ± SD, *P < 0.05, ***P < 0.001, ****P < 0.0001, n = 4 compared to each other group by one-way ANOVA.
Techniques Used: SDS Page, Staining, Enzyme-linked Immunosorbent Assay, Labeling, Flow Cytometry, Binding Assay, Cell Culture, Expressing, Bioprocessing, Co-Culture Assay
Related Articles
Labeling:Article Title: The combination of LILRB4-targeting NK cell engagers and cGAS–STING agonists enhances the anti–multiple myeloma immune activity of NK cells Article Snippet: Briefly, BiKE and the control monoclonal antibody (20 μg/ml) were incubated with cells for 1 hour at room temperature, washed twice with PBS, and incubated with FITC-labeled goat anti-human IgG (H + L) secondary antibody (1:1000, Cat: SA00003−12, Proteintech, China) for 20 minutes at room temperature. .. Additionally, the same indirect labeling method was used to detect LILRB4 expression on MM cell membranes by flow cytometry, using Article Title: The combination of LILRB4-targeting NK cell engagers and cGAS-STING agonists enhances the anti-multiple myeloma immune activity of NK cells. Article Snippet: Briefly, BiKE and the control monoclonal antibody (20 μg/ml) were incubated with cells for 1 hour at room temperature, washed twice with PBS, and incubated with FITC-labeled goat anti-human IgG (H + L) secondary antibody (1:1000, Cat: SA00003−12, Proteintech, China) for 20 minutes at room temperature. .. Additionally, the same indirect labeling method was used to detect LILRB4 expression on MM cell membranes by flow cytometry, using Expressing:Article Title: The combination of LILRB4-targeting NK cell engagers and cGAS–STING agonists enhances the anti–multiple myeloma immune activity of NK cells Article Snippet: Briefly, BiKE and the control monoclonal antibody (20 μg/ml) were incubated with cells for 1 hour at room temperature, washed twice with PBS, and incubated with FITC-labeled goat anti-human IgG (H + L) secondary antibody (1:1000, Cat: SA00003−12, Proteintech, China) for 20 minutes at room temperature. .. Additionally, the same indirect labeling method was used to detect LILRB4 expression on MM cell membranes by flow cytometry, using Article Title: The combination of LILRB4-targeting NK cell engagers and cGAS-STING agonists enhances the anti-multiple myeloma immune activity of NK cells. Article Snippet: Briefly, BiKE and the control monoclonal antibody (20 μg/ml) were incubated with cells for 1 hour at room temperature, washed twice with PBS, and incubated with FITC-labeled goat anti-human IgG (H + L) secondary antibody (1:1000, Cat: SA00003−12, Proteintech, China) for 20 minutes at room temperature. .. Additionally, the same indirect labeling method was used to detect LILRB4 expression on MM cell membranes by flow cytometry, using Flow Cytometry:Article Title: The combination of LILRB4-targeting NK cell engagers and cGAS–STING agonists enhances the anti–multiple myeloma immune activity of NK cells Article Snippet: Briefly, BiKE and the control monoclonal antibody (20 μg/ml) were incubated with cells for 1 hour at room temperature, washed twice with PBS, and incubated with FITC-labeled goat anti-human IgG (H + L) secondary antibody (1:1000, Cat: SA00003−12, Proteintech, China) for 20 minutes at room temperature. .. Additionally, the same indirect labeling method was used to detect LILRB4 expression on MM cell membranes by flow cytometry, using Article Title: The combination of LILRB4-targeting NK cell engagers and cGAS-STING agonists enhances the anti-multiple myeloma immune activity of NK cells. Article Snippet: Briefly, BiKE and the control monoclonal antibody (20 μg/ml) were incubated with cells for 1 hour at room temperature, washed twice with PBS, and incubated with FITC-labeled goat anti-human IgG (H + L) secondary antibody (1:1000, Cat: SA00003−12, Proteintech, China) for 20 minutes at room temperature. .. Additionally, the same indirect labeling method was used to detect LILRB4 expression on MM cell membranes by flow cytometry, using |
